Cities in fiction: Perambulations with John Berger

This paper explores selected novels by John Berger in which cities play a central role. These cities are places, partially real and partially imagined, where memory, hope, and despair intersect. My reading of the novels enables me to trace important themes in recent discourses on the nature of contemporary capitalism, including notions of resistance and universality. I also show how Berger?s work points to a writing that can break free from the curious capacity of capitalism to absorb and feed of its critique

Sparse channel estimation for multicarrier underwater acoustic communication : from subspace methods to compressed sensing

Author Posting. © IEEE, 2009. This article is posted here by permission of IEEE for personal use, not for redistribution. The definitive version was published in IEEE Transactions on Signal Processing 58 (2010): 1708-1721, doi:10.1109/TSP.2009.2038424.In this paper, we investigate various channel estimators that exploit channel sparsity in the time and/or Doppler domain for a multicarrier underwater acoustic system. We use a path-based channel model, where the channel is described by a limited number of paths, each characterized by a delay, Doppler scale, and attenuation factor, and derive the exact inter-carrierinterference (ICI) pattern. For channels that have limited Doppler spread we show that subspace algorithms from the array processing literature, namely Root-MUSIC and ESPRIT, can be applied for channel estimation. For channels with Doppler spread, we adopt a compressed sensing approach, in form of Orthogonal Matching Pursuit (OMP) and Basis Pursuit (BP) algorithms, and utilize overcomplete dictionaries with an increased path delay resolution. Numerical simulation and experimental data of an OFDM block-by-block receiver are used to evaluate the proposed algorithms in comparison to the conventional least-squares (LS) channel estimator.We observe that subspace methods can tolerate small to moderate Doppler effects, and outperform the LS approach when the channel is indeed sparse. On the other hand, compressed sensing algorithms uniformly outperform the LS and subspace methods. Coupled with a channel equalizer mitigating ICI, the compressed sensing algorithms can effectively handle channels with significant Doppler spread.C. Berger, S. Zhou, and P. Willett are supported by ONR grants N00014-09-10613, N00014-07-1-0805, and N00014-09-1-0704

FACS purification and transcriptome analysis of drosophila neural stem cells reveals a role for Klumpfuss in self-renewal

Drosophila neuroblasts (NBs) have emerged as a model for stem cell biology that is ideal for genetic analysis but is limited by the lack of cell-type-specific gene expression data. Here, we describe a method for isolating large numbers of pure NBs and differentiating neurons that retain both cell-cycle and lineage characteristics. We determine transcriptional profiles by mRNA sequencing and identify 28 predicted NB-specific transcription factors that can be arranged in a network containing hubs for Notch signaling, growth control, and chromatin regulation. Overexpression and RNA interference for these factors identify Klumpfuss as a regulator of self-renewal. We show that loss of Klumpfuss function causes premature differentiation and that overexpression results in the formation of transplantable brain tumors. Our data represent a valuable resource for investigating Drosophila developmental neurobiology, and the described method can be applied to other invertebrate stem cell lineages as well

Impact of elosulfase alfa in patients with morquio A syndrome who have limited ambulation: An open-label, phase 2 study.

Efficacy and safety of elosulfase alfa enzyme replacement therapy (ERT) were assessed in an open-label, phase 2, multi-national study in Morquio A patients aged ≥5 years unable to walk ≥30 meters in the 6-min walk test. Patients received elosulfase alfa 2.0 mg/kg/week intravenously for 48 weeks. Efficacy measures were functional dexterity, pinch/grip strength, mobility in a modified timed 25-foot walk, pain, quality of life, respiratory function, and urine keratan sulfate (KS). Safety/tolerability was also assessed. Fifteen patients received elosulfase alfa, three patients discontinued ERT due to adverse events (two were grade 3 drug-related adverse events, the other was not drug-related), and two patients missed >20% of planned infusions; 10 completed treatment through 48 weeks and received ≥80% of planned infusions (Modified Per Protocol [MPP] population). The study population had more advanced disease than that enrolled in other trials. From baseline to week 48, MPP data showed biochemical efficacy (urine KS decreased 52.4%). The remaining efficacy results were highly variable due to challenges in test execution because of severe skeletal and joint abnormalities, small sample sizes, and clinical heterogeneity among patients. Eight patients showed improvements in one or more outcome measures; several patients indicated improvements not captured by the study assessments (e.g., increased energy, functional ability). The nature of adverse events was similar to other elosulfase alfa studies. This study illustrates the considerable challenges in objectively measuring impact of ERT in very disabled Morquio A patients and highlights the need to examine results on an individual basis. © 2016 The Authors. American Journal of Medical Genetics Part A Published by Wiley Periodicals, Inc